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Somaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers

cris.virtual.author-orcid0000-0003-3721-6473
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cris.virtual.author-orcid0000-0002-2222-6866
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cris.virtualsource.author-orcid09c81af2-c2b2-424c-b2ec-4cda600883bf
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dc.abstract.enStarch content is one of the major quality criteria targeted by potato breeding programs. Traditional potato breeding is a laborious duty due to the tetraploid nature and immense heterozygosity of potato genomes. In addition, screening for functional genetic variations in wild relatives is slow and strenuous. Moreover, genetic diversity, which is the raw material for breeding programs, is limited due to vegetative propagation used in the potato industry. Somaclonal variation provides a time-efficient tool to breeders for obtaining genetic variability, which is essential for breeding programs, at a reasonable cost and independent of sophisticated technology. The present investigation aimed to create potato somaclones with an improved potential for starch accumulation. Based on the weight and starch content of tubers, the somaclonal variant Ros 119, among 105 callus-sourced clones, recorded a higher tuberization potential than the parent cv Lady Rosetta in a field experiment. Although this somaclone was similar to the parent in the number of tubers produced, it exhibited tubers with 42 and 61% higher fresh and dry weights, respectively. Additionally, this clone recorded 10 and 75% increases in starch content based on the dry weight and average content per plant, respectively. The enhanced starch accumulation was associated with the upregulation of six starch-synthesis-related genes, namely, the AGPase, GBSS I, SBE I, SBE II, SS II and SS III genes. AGPase affords the glycosyl moieties required for the synthesis of amylose and amylopectin. GBSS is required for amylose elongation, while SBE I, SBE II, SS II and SS III are responsible for amylopectin.
dc.affiliationWydział Inżynierii Środowiska i Inżynierii Mechanicznej
dc.affiliation.instituteKatedra Inżynierii Biosystemów
dc.contributor.authorAdly, Walaa M. R. M.
dc.contributor.authorNiedbała, Gniewko
dc.contributor.authorEL-Denary, Mohammad E.
dc.contributor.authorMohamed, Mahasen A.
dc.contributor.authorPiekutowska, Magdalena
dc.contributor.authorWojciechowski, Tomasz
dc.contributor.authorAbd El-Salam, El-Sayed T.
dc.contributor.authorFouad, Ahmed S.
dc.date.access2025-08-21
dc.date.accessioned2025-09-04T11:45:00Z
dc.date.available2025-09-04T11:45:00Z
dc.date.copyright2023-01-04
dc.date.issued2023
dc.description.abstract<jats:p>Starch content is one of the major quality criteria targeted by potato breeding programs. Traditional potato breeding is a laborious duty due to the tetraploid nature and immense heterozygosity of potato genomes. In addition, screening for functional genetic variations in wild relatives is slow and strenuous. Moreover, genetic diversity, which is the raw material for breeding programs, is limited due to vegetative propagation used in the potato industry. Somaclonal variation provides a time-efficient tool to breeders for obtaining genetic variability, which is essential for breeding programs, at a reasonable cost and independent of sophisticated technology. The present investigation aimed to create potato somaclones with an improved potential for starch accumulation. Based on the weight and starch content of tubers, the somaclonal variant Ros 119, among 105 callus-sourced clones, recorded a higher tuberization potential than the parent cv Lady Rosetta in a field experiment. Although this somaclone was similar to the parent in the number of tubers produced, it exhibited tubers with 42 and 61% higher fresh and dry weights, respectively. Additionally, this clone recorded 10 and 75% increases in starch content based on the dry weight and average content per plant, respectively. The enhanced starch accumulation was associated with the upregulation of six starch-synthesis-related genes, namely, the AGPase, GBSS I, SBE I, SBE II, SS II and SS III genes. AGPase affords the glycosyl moieties required for the synthesis of amylose and amylopectin. GBSS is required for amylose elongation, while SBE I, SBE II, SS II and SS III are responsible for amylopectin.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,0
dc.description.number2
dc.description.points70
dc.description.versionfinal_published
dc.description.volume12
dc.identifier.doi10.3390/plants12020232
dc.identifier.issn2223-7747
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4638
dc.identifier.weblinkhttps://www.mdpi.com/2223-7747/12/2/232
dc.languageen
dc.relation.ispartofPlants
dc.relation.pagesart. 232
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enpotato
dc.subject.entissue culture
dc.subject.ensomaclonal variation
dc.subject.enstarch
dc.subject.engene expression
dc.titleSomaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers
dc.title.volumeSpecial Issue Plant Biotechnology and Crop Improvement
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue2
oaire.citation.volume12