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Expression and localization of the neuropeptide phoenixin-14 and its receptor GRP173 in the canine reproductive organs and periovarian adipose tissue

cris.virtual.author-orcid0000-0002-4563-3798
cris.virtual.author-orcid0000-0002-2198-6046
cris.virtual.author-orcid0000-0003-4059-1362
cris.virtual.author-orcid0000-0002-3651-0972
cris.virtual.author-orcid0000-0001-5046-7416
cris.virtual.author-orcid0000-0002-1820-5202
cris.virtual.author-orcid0000-0003-4961-2959
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid2887b0c9-4f57-4235-a68f-057f9a3211f9
cris.virtualsource.author-orcid2068f136-150d-448d-a014-c5b41eb5cb60
cris.virtualsource.author-orcid9118c61a-b066-4efc-a7af-486f9ebf9021
cris.virtualsource.author-orcid652e209d-434a-47b3-b5fb-64c0aac5a84c
cris.virtualsource.author-orcid8cc943c4-fa09-421d-9e2b-84b33ec18db9
cris.virtualsource.author-orcid9fd4d04e-0912-425f-812f-aab2e3debe36
cris.virtualsource.author-orcidd262a047-0e71-4e00-b46b-5167dd661a3d
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enPhoenixin-14 (PNX-14) is a regulatory neuropeptide encoded by the SMIM20 gene, which has been implicated in the reproductive cycle by modulating the hypothalamic-pituitary-gonadal (HPG) axis. Recently, we showed that PNX-14 is downregulated in bitches with cystic endometrial hyperplasia and pyometra. The objective of this study was to determine the expression of Smim20, PNX-14, and its putative receptor GRP173 in the canine ovary (both healthy and those with ovarian cysts), periovarian adipose tissue (PAT) and in the endometrium during the oestrous cycle. The expression was analysed by RT-qPCR and Western blot. In tissue sections, peptides were localised by immunofluorescent assays, and blood plasma concentrations of PNX-14 were detected by EIA. The results demonstrated increased levels of PNX in bitches in the anestrus groups compared to diestrus animals. The expression of GPR173 increased in PAT during the diestrus phase and endometrial tissue in late diestrus bitches. In the ovary, strong signals of PNX-14 and GPR173 were detected in the luteal and follicular cells. Furthermore, bitches with cystic ovaries were characterised by elevated circulating PNX levels and a significantly higher expression of PNX and GPR173 in gonadal tissues, when compared with healthy animals. Moreover, a positive correlation between PNX and progesterone in the blood of healthy bitches was noted, which changed to a negative correlation in females affected by cystic ovaries. These studies expand the knowledge regarding the expression and localization of the PNX/GRP173 system in canine reproductive organs during physiological and pathological conditions.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Nauk Przedklinicznych i Chorób Zakaźnych
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.affiliation.instituteKatedra Chorób Wewnętrznych i Diagnostyki
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.contributor.authorRybska, Marta
dc.contributor.authorBillert, Maria
dc.contributor.authorSkrzypski, Marek
dc.contributor.authorWojciechowicz, Tatiana
dc.contributor.authorKubiak, Magdalena
dc.contributor.authorŁukomska, Anna
dc.contributor.authorNowak, Tomasz
dc.contributor.authorWłodarek, Jan
dc.contributor.authorWąsowska, Barbara
dc.date.access2025-05-30
dc.date.accessioned2025-09-02T07:40:25Z
dc.date.available2025-09-02T07:40:25Z
dc.date.copyright2023-06-19
dc.date.issued2023
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,2
dc.description.numberAugust 2023
dc.description.points140
dc.description.versionfinal_published
dc.description.volume255
dc.identifier.doi10.1016/j.anireprosci.2023.107282
dc.identifier.eissn1873-2232
dc.identifier.issn0378-4320
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4571
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0378432023000969?via%3Dihub
dc.languageen
dc.relation.ispartofAnimal Reproduction Science
dc.relation.pagesart. 107282
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.encanine
dc.subject.enovary
dc.subject.enperiovarian adipose tissue
dc.subject.enuterine
dc.subject.enphoenixin
dc.subject.enGPR173 receptor
dc.titleExpression and localization of the neuropeptide phoenixin-14 and its receptor GRP173 in the canine reproductive organs and periovarian adipose tissue
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume255