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Simple Method for Assessing Diversity and Dynamics of Microbial Community: Comparison of Dairy Phages from Industrial and Spontaneous Fermentation

cris.virtual.author-orcid0000-0002-5418-5632
cris.virtual.author-orcid0000-0001-6691-4444
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cris.virtual.author-orcid0000-0001-8417-2245
cris.virtualsource.author-orcidccd52a90-8355-4384-b6ea-c94578ebf2ce
cris.virtualsource.author-orcidb751905b-aeac-4819-b01d-045ff92e0493
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cris.virtualsource.author-orcidedf63522-bb89-4788-a8d3-9c3f3858875b
dc.abstract.enBackground: The dairy industry heavily relies on fermentation processes driven in high proportion by Lactococcus lactis. The fermentation process can be perturbed or even stopped by bacteriophage activity leading to complete loss of fermentation batch or decreased quality product. Monitoring of the phage diversity and dynamics in the process allows to implement protective measures (e.g. starter rotation) in order to maintain unperturbed production.; Methods: Universal primers were used to amplify sequences of the 936, c2, and P335 Lactococcus phage types. The amplicons were sequences with Sanger method and obtained degenerate sequences were analyzed using simple bioinformatic pipeline in R environment.; Results: The most prevalent phage type is 936, followed by P335, whereas c2 type is less frequent.; Conclusions: Curd cheeses prepared on non-pasteurized milk based on native milk microbiota had higher diversity of phages distinct of these found in dairy plants. Sanger sequencing of heterogenous amplicons generated on metagenome DNA can be used to asses low-complexity microbiota diversity.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.contributor.authorOlejnik-Schmidt, Agnieszka
dc.contributor.authorPietrzak, Bernadeta
dc.contributor.authorKawacka, Iwona
dc.contributor.authorMalak, Klaudia
dc.contributor.authorWawrzyniak, Weronika
dc.contributor.authorSchmidt, Marcin
dc.date.access2025-10-16
dc.date.accessioned2026-02-18T12:05:46Z
dc.date.available2026-02-18T12:05:46Z
dc.date.copyright2021-08-19
dc.date.issued2021
dc.description.abstract<jats:p>Background: The dairy industry heavily relies on fermentation processes driven in high proportion by Lactococcus lactis. The fermentation process can be perturbed or even stopped by bacteriophage activity leading to complete loss of fermentation batch or decreased quality product. Monitoring of the phage diversity and dynamics in the process allows to implement protective measures (e.g. starter rotation) in order to maintain unperturbed production.; Methods: Universal primers were used to amplify sequences of the 936, c2, and P335 Lactococcus phage types. The amplicons were sequences with Sanger method and obtained degenerate sequences were analyzed using simple bioinformatic pipeline in R environment.; Results: The most prevalent phage type is 936, followed by P335, whereas c2 type is less frequent.; Conclusions: Curd cheeses prepared on non-pasteurized milk based on native milk microbiota had higher diversity of phages distinct of these found in dairy plants. Sanger sequencing of heterogenous amplicons generated on metagenome DNA can be used to asses low-complexity microbiota diversity.</jats:p>
dc.description.accesstimebefore_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.versionfinal_author
dc.identifier.doi10.20944/preprints202108.0397.v1
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/7380
dc.identifier.weblinkhttps://www.preprints.org/manuscript/202108.0397/v1
dc.languageen
dc.relation.ispartofPreprints.org
dc.relation.pagesart. 2021080397
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_REPOSITORY
dc.subject.enphage
dc.subject.endairy
dc.subject.enLactococcus
dc.subject.endiversity
dc.subject.encommunity
dc.subtypeArticleEarlyAccess
dc.titleSimple Method for Assessing Diversity and Dynamics of Microbial Community: Comparison of Dairy Phages from Industrial and Spontaneous Fermentation
dc.typeJournalArticle
dspace.entity.typePublication