Simple item page
 

Effect of Vicia sativa L. on Motility, Mortality and Expression Levels of hsp Genes in J2 Stage of Meloidogyne hapla

cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-0102-0084
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid51a5a68b-106b-4e9d-bd9b-79d15d3ec0c1
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enAssuming that the seeds of Vicia sativa L. have a stressful effect on J2 stage Meloidogyne hapla, we undertook research on the effect of these seeds on the motility and mortality of J2 and determined the expression levels of selected hsp genes in J2. The assessment of the effect of V. sativa seeds on the motility of M. hapla specimens consisted of observing the movement of J2 immersed in a seed diffusate or in a tomato root filtrate at temperatures of 10, 17, and 21°C. In J2 treated with V. sativa (cv. Ina) seed diffusates, the expression level of hsp genes was determined by qPCR. J2 exposed to V. sativa diffusates were found to lose their motility, while their mortality did not exceed 30%. J2 in the seed diffusate were characterized by an increase in the expression levels of the Mh-hsp90, Mh-hsp1, and Mh-hsp43 genes. It is suggested that the hsp90 gene may be a potential bioindicator of the environmental impact on Meloidogyne nematodes. The impaired ability to move in J2 of M. hapla is attributable to the occurrence of V. sativa seeds in their habitat. These studies may contribute to developing methods of reducing crop damage caused by M. hapla.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Metod Matematycznych i Statystycznych
dc.contributor.authorDobosz, Renata
dc.contributor.authorFlis, Łukasz
dc.contributor.authorBocianowski, Jan
dc.contributor.authorMalewski, Tadeusz
dc.date.access2023-04-14
dc.date.accessioned2025-08-29T11:28:22Z
dc.date.available2025-08-29T11:28:22Z
dc.date.copyright2023-04-14
dc.date.issued2023
dc.description.abstract<jats:title>Abstract</jats:title> <jats:p>Assuming that the seeds of <jats:italic>Vicia sativa</jats:italic> L. have a stressful effect on J2 stage <jats:italic>Meloidogyne hapla</jats:italic>, we undertook research on the effect of these seeds on the motility and mortality of J2 and determined the expression levels of selected <jats:italic>hsp</jats:italic> genes in J2. The assessment of the effect of <jats:italic>V. sativa</jats:italic> seeds on the motility of <jats:italic>M. hapla</jats:italic> specimens consisted of observing the movement of J2 immersed in a seed diffusate or in a tomato root filtrate at temperatures of 10, 17, and 21°C. In J2 treated with <jats:italic>V. sativa</jats:italic> (cv. Ina) seed diffusates, the expression level of <jats:italic>hsp</jats:italic> genes was determined by qPCR. J2 exposed to <jats:italic>V. sativa</jats:italic> diffusates were found to lose their motility, while their mortality did not exceed 30%. J2 in the seed diffusate were characterized by an increase in the expression levels of the <jats:italic>Mh-hsp</jats:italic>90, <jats:italic>Mh-hsp</jats:italic>1, and <jats:italic>Mh-hsp</jats:italic>43 genes. It is suggested that the <jats:italic>hsp</jats:italic>90 gene may be a potential bioindicator of the environmental impact on <jats:italic>Meloidogyne</jats:italic> nematodes. The impaired ability to move in J2 of <jats:italic>M. hapla</jats:italic> is attributable to the occurrence of <jats:italic>V. sativa</jats:italic> seeds in their habitat. These studies may contribute to developing methods of reducing crop damage caused by <jats:italic>M. hapla</jats:italic>.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if1,4
dc.description.number1
dc.description.points100
dc.description.versionfinal_published
dc.description.volume55
dc.identifier.doi10.2478/jofnem-2023-0009
dc.identifier.eissn2640-396X
dc.identifier.issn0022-300X
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4528
dc.identifier.weblinkhttps://sciendo.com/pl/article/10.2478/jofnem-2023-0009
dc.languageen
dc.pbn.affiliationagriculture and horticulture
dc.relation.ispartofJournal of Nematology
dc.relation.pagese2023-1
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enbehavior
dc.subject.enhsp genes
dc.subject.enMeloidogyne hapla
dc.subject.enmotility
dc.subject.enmortality
dc.subject.enVicia sativa seeds
dc.titleEffect of Vicia sativa L. on Motility, Mortality and Expression Levels of hsp Genes in J2 Stage of Meloidogyne hapla
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume55