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The Contribution of Cornelian Cherry (Cornus mas L.) Alcoholic Beverages on the Sensory, Nutritional and Anti-Nutritional Characteristics—In Vitro and In Silico Approaches

2024, Szczepaniak, Oskar, Stachowiak, Barbara, Jeleń, Henryk, Stuper-Szablewska, Kinga, Szambelan, Katarzyna Teresa, Kobus-Cisowska, Joanna

Food producers have focused on novel and attractive raw materials with functional properties. Cornelian cherry (Cornus mas L.) fruits contain numerous compounds that may be beneficial for health. Objective: This study aimed to compare and assess the physicochemical properties and amygdalin levels in brandy and liquor prepared from frozen cornelian cherry fruits. Density functional theory-based B3LYP functionals were used to analyze the spectral and optical properties of amygdalin. The contents of the compounds and volatile products of amygdalin decay were found in two spirituose beverages of Cornus mas, using HPLC and GC-MS. Significant differences in their physicochemical properties were detected between the samples. Alcoholic beverages based on cornelian cherry fruits were rich in a wide range of functional ingredients with a low concentration of amygdalin. In silico analysis showed that orbital density diffusion has a major effect on the physical properties of amygdalin, while differences between the polarities of water and ethanol had no noticeable effect on the spectral properties of the compound. Cornelian cherry-based alcoholic drinks might be interesting functional products with rich aromatic bouquets. The amygdalin concentration is low enough to pose no toxicological threat, but rather shapes the tastory bouquet of the products. Levels of amygdalin may be controlled using the same analytical methods for solutions with different ethanol–water ratios.

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Biochemical Properties of Bioactive Compounds in the Oil from Polish Varieties of Camelina sativa Cultivated in 2019–2022

2024, Przybylska-Balcerek, Anna, Kurasiak-Popowska, Danuta, Graczyk, Małgorzata, Szczepańska-Alvarez, Anna, Rzyska, Katarzyna, Stuper-Szablewska, Kinga

AbstractCold‐pressed Camelina oil is a traditional oil registered as a traditional food in Poland. Camelina oil has health‐promoting properties and high oxidative stability. This may be due to the presence of various bioactive antioxidant compounds such as carotenoids, sterols and polyphenols. Bioactive compounds content in Camelina oil depends mainly on the varieties and on the conditions under which the crop was grown therefore the aim of the research was to analyse antioxidant bioactive compounds in oil from different cultivars of Camelina sativa seeds and to determine their relationship with oil parameters.

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Profile of Polyphenols, Fatty Acids, and Terpenes in Henola Hemp Seeds Depending on the Method of Fertilization

2024, Przybylska-Balcerek, Anna, Frankowski, Jakub, Graczyk, Małgorzata, Niedziela, Grażyna, Sieracka, Dominika, Wacławek, Stanisław, Sázavská, Tereza Hulswit, Buśko, Maciej, Szwajkowska-Michałek, Lidia, Stuper-Szablewska, Kinga

Botanical varieties of hemp differ in chemical composition, plant morphology, agronomy, and industrial suitability. Hemp is popular for cultivation for the production of cannabinoid oil, fiber production, biomass, etc. The fertilization process is one of the most important factors affecting the plant, both its condition and chemical composition. So far, research has been carried out proving that hemp is a valuable source of, among others: fatty acids, amino acids, acids, vitamins, numerous micro- and macroelements, and antioxidant compounds. In this experiment, it was decided to check the possibility of harvesting hemp panicles twice in one year. The purpose of this treatment is to use one plant to produce cannabidiol oil and grain. The main aim of the research was to determine bioactive compounds in hemp seeds and to determine whether the cultivation method affects their content and quantity. Based on the research conducted, it was observed that hemp can be grown in two directions at the same time and harvested twice because its health-promoting properties do not lose their value. It was found that regardless of whether hemp is grown solely for seeds or to obtain essential oils and then seeds, the type of fertilization does not affect the content of phenolic acids (e.g., syringic acid: 69.69–75.14 μg/100 g, vanillic acid: 1.47–1.63 μg/100 g). Based on the conducted research, it was found that essential oils can be obtained from one plant in the summer and seeds from Henola hemp cultivation in the autumn, because such a treatment does not affect the content of the discussed compounds.

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Camelina sativa Seeds and Oil as Ingredients in Model Muffins in Order to Enhance Their Health-Promoting Value

2024, Bilska, Agnieszka, Kurasiak-Popowska, Danuta, Szablewski, Tomasz, Radzimirska-Graczyk, Monika, Stuper-Szablewska, Kinga

The aim of this study was to see whether it is possible to add camelina oil and seeds as ingredients in muffins in order to enhance their health-promoting value, such as their bioactive compound content, while maintaining the organoleptic attributes considered desirable by consumers. Camelina oil is characterised by a high linolenic acid content. Four types of muffins were prepared for analysis: MBnO—control muffins (containing 11.85% rapeseed oil), MCsO—muffins containing camelina oil instead of rapeseed oil, MCsS—muffins containing 6.65% camelina seeds in relation to the mass of prepared dough, and MCsOS—muffins containing both camelina oil and camelina seeds. The change in the fatty acid profile in muffins with the addition of camelina oil was significant; however, it was found that, as a result of thermal treatment, lower amounts of saturated fatty acids were formed. Among all the investigated experimental variants, muffins were characterised by the highest contents of all the phenolic acids analysed. The substitution of rapeseed oil with camelina oil had no negative effect on most of the organoleptic attributes of the muffins. Moreover, thanks to a greater content of carotenoids, camelina oil had an advantageous effect on the improvement of product colour, thus improving its overall desirability.

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Impact of Various Essential Oils on the Development of Pathogens of the Fusarium Genus and on Health and Germination Parameters of Winter Wheat and Maize

2024, Danielewicz, Jakub, Grzanka, Monika, Sobiech, Łukasz, Jajor, Ewa, Horoszkiewicz, Joanna, Korbas, Marek, Blecharczyk, Andrzej, Stuper-Szablewska, Kinga, Matysiak, Kinga

Currently, researchers are looking for ways to replace synthetic pesticides with substances of natural origin. Essential oils are produced by plants, among other things, to protect against pathogens, which is why there is interest in their use as fungicides. This experiment assessed the composition of essential oils from a commercial source, their impact on the development of mycelium of pathogens of the Fusarium genus, and the possibility of using them as a pre-sowing treatment. Grains of winter wheat (Triticum aestivum L.) and corn (Zea mays L.) were inoculated with a suspension of mycelium and spores of fungi of the Fusarium genus and then soaked in solutions containing oils of sage (Salvia officinalis L.), cypress (Cupressus sempervirens L.), cumin (Cuminum cyminum L.), and thyme (Thymus vulgaris L.). The obtained results indicate that thyme essential oil had the strongest effect on limiting the development of Fusarium pathogens and seedling infection, but at the same time it had an adverse effect on the level of germination and seedling development of the tested plants. The remaining essential oils influenced the mentioned parameters to varying degrees. Selected essential oils can be an alternative to synthetic fungicides, but they must be selected appropriately.

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Activated Carbon from Coconut Shells as a Modifier of Urea–Formaldehyde Resin in Particleboard Production

2024, Kawalerczyk, Jakub, Dukarska, Dorota, Antov, Petar, Stuper-Szablewska, Kinga, Dziurka, Dorota, Mirski, Radosław

Various methods for the effective modification of urea–formaldehyde (UF) adhesives, aimed at enhancing the performance of wood-based materials, have been continually explored worldwide. The aim of this work was to investigate and evaluate the effect of introducing small amounts (0.25–1.5%) of activated carbon from coconut shells (ACCS) in UF adhesive on the properties of particleboard. The performed investigations of the adhesive mixture’s properties showed an increase in both viscosity and reactivity. Moreover, the use of loadings of 0.75% and 1% had a positive effect on mechanical properties such as bending strength, modulus of elasticity, and internal bond. In these variants, a delay in the degradation of the adhesive bonds by water was also observed, as indicated by the lower thickness swelling values measured after 2 h. However, under long-term exposure to water, the modification had no considerable effect on the dimensional stability of the boards. Markedly, the addition of 1 and 1.5% of ACCS resulted in a reduction in formaldehyde content, which can be attributed to the excellent adsorption capacity of activated carbon. Overall, a loading of 1% was found to be optimal, resulting in improved strength, enhanced water resistance, and reduced formaldehyde content.

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Wpływ wybranych herbicydów na rozwój Camelina sativa L. Crantz i Brassica carinata L. Brown

2024, Grzanka, Monika, Piechota, Tomasz, Kurasiak-Popowska, Danuta, Stuper-Szablewska, Kinga, Glina, Bartłomiej, Mikołajczyk, Sylwia, Tomkowiak, Agnieszka, Rzyska-Szczupak, Katarzyna, Buśko, Maciej

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Docking analysis of phenolic acid and flavonoids with selected TAS2R receptors and in vitro experiment

2024, Szczepaniak, Oskar, Jokiel, Maria, Stuper-Szablewska, Kinga, Kobus-Cisowska, Joanna

AbstractCornelian cherry fruits contain a wide range of phenolic acids, flavonoids, and other secondary metabolites. Selected flavonoids may inhibit the perceiving of bitterness, however, the full mechanism with all TAS2R bitter taste receptors is not known. The aim of the study was to determine the inhibitory effect of Cornus mas phenolics against the bitterness receptors TAS2R13 and TAS2R3 through functional in vitro assays and coupling studies. The overall effect was validated by analysing the inhibition of the receptors activity in cells treated with tested cornelian cherry extracts. The strength of interaction with both TAS2R receptors varied between studied compounds with different binding affinity. Most compounds bonded with the TAS2R3 receptor through a long-distant hydrophobic interaction with Trp89A and π–π orbital overlapping—between phenolic and tryptophane aromatic rings. For TAS2R13 observed were various mechanisms of interaction with the compounds. Nonetheless, naringin and quercetin had most similar binding affinity to chloroquine and denatonium—the model agonists for the receptor.

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Assessment of the Antioxidative Properties of Extracts from the Fruits of Pyrus pyraster (L.) Burgsd and Pyrus ×myloslavensis Czarna & Antkowiak Grown under Natural Environmental Conditions

2024, Hęś, Marzanna, Antkowiak, Wojciech, Stuper-Szablewska, Kinga, Dziedzic, Krzysztof, Jessa, Marta, Ratajczak, Paulina

Analyses were conducted on extracts from the fruits of P. pyraster and P. ×myloslavensis. Extraction with 80% methanol was performed at room temperature. The total phenolic content was determined by spectrophotometry using the Folin–Ciocalteu reagent, with gallic acid as the reference standard. Phenolic compounds and organic acids were identified on a liquid chromatograph. The antioxidative activity of the extracts was tested in relation to linoleic acid incubation of the emulsions for 19 h based on the neutralization of the DPPH radical (2,2-diphenyl-1-picrylhydrazyl) and the ABTS cation radical (2,2′-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid]) as well as by the ferric reducing antioxidant power (FRAP) assay. The analyses showed that the extract from P. pyraster fruits is characterized by a higher content of phenolic compounds and a higher antioxidative potential compared with that from P. ×myloslavensis. In extracts of both pear species, seven phenolic compounds and four organic acids were identified. The total fiber content in pears of P. pyraster and P. ×myloslavensis was determined at 36.45 g and 24.74 g/100 g d.m. of the pear fruits, of which most comprised the insoluble fraction (32.49 g and 20.86/100 g, respectively). The results of the conducted research are highly significant, as they confirm that pears contain many valuable nutrients and biologically active compounds, including antioxidants and dietary fiber. Adding pear extracts to food products may offer a way to boost their health benefits while also broadening the variety of items that have appealing sensory characteristics. Moreover, research has shown that fruit extracts can help to prolong the shelf life of food products by safeguarding them against lipid oxidation and the decline in their nutritional value.