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Co-Dispersion Delivery Systems with Solubilizing Carriers Improving the Solubility and Permeability of Cannabinoids (Cannabidiol, Cannabidiolic Acid, and Cannabichromene) from Cannabis sativa (Henola Variety) Inflorescences
Cannabinoids: cannabidiol (CBD), cannabidiolic acid (CBDA), and cannabichromene (CBC) are lipophilic compounds with limited water solubility, resulting in challenges related to their bioavailability and therapeutic efficacy upon oral administration. To overcome these limitations, we developed co-dispersion cannabinoid delivery systems with the biopolymer polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol (Soluplus) and magnesium aluminometasilicate (Neusilin US2) to improve solubility and permeability. Recognizing the potential therapeutic benefits arising from the entourage effect, we decided to work with an extract instead of isolated cannabinoids. Cannabis sativa inflorescences (Henola variety) with a confirming neuroprotective activity were subjected to dynamic supercritical CO2 (scCO2) extraction and next they were combined with carriers (1:1 mass ratio) to prepare the co-dispersion cannabinoid delivery systems (HiE). In vitro dissolution studies were conducted to evaluate the solubility of CBD, CBDA, and CBC in various media (pH 1.2, 6.8, fasted, and fed state simulated intestinal fluid). The HiE-Soluplus delivery systems consistently demonstrated the highest dissolution rate of cannabinoids. Additionally, HiE-Soluplus exhibited the highest permeability coefficients for cannabinoids in gastrointestinal tract conditions than it was during the permeability studies using model PAMPA GIT. All three cannabinoids exhibited promising blood-brain barrier (BBB) permeability (Papp higher than 4.0 × 10−6 cm/s), suggesting their potential to effectively cross into the central nervous system. The improved solubility and permeability of cannabinoids from the HiE-Soluplus delivery system hold promise for enhancement in their bioavailability.
Evaluation of the Effect of Conventional and Stabilized Nitrogen Fertilizers on the Nutritional Status of Several Maize Cultivars (Zea mays L.) in Critical Growth Stages Using Plant Analysis
The study presents the results of a three year field trial aimed at assessing the nutritional status of maize in critical growth stages by means of a plant analysis in the cultivation of three maize cultivars differing in their agronomic and genetic profile. The main research problem was to demonstrate whether the availability of nitrogen from stabilized fertilizers for “stay-green” maize varieties is consistent with the dynamics of the demand for this component. This is very important from both the economic and agronomic aspect of maize cultivation. The research showed a significant response of the maize cultivars to different nitrogen fertilizer formulations, which was observed in the period from the five-leaf stage to the full flowering stage. The advantage of the fertilizer, UltraGran stabilo, over other nitrogen fertilizers in the BBCH 15 stage was demonstrated only for the cultivar, ES Metronom, which produced a greater aerial mass while maintaining the nitrogen concentration at the level of the other two maize cultivars. The nitrogen and potassium content shaped the kernel weight in the ear in the flowering stage, confirming the importance of the interaction of these two elements in forming this feature of maize as the main predictor of the grain yield. This trait (expressed by the R2 coefficient) manifested each year of the study, but especially in the years with optimal weather patterns (i.e., the first year). The response of the maize cultivars to nitrogen fertilizers, especially the cultivar, ES Metronom, was manifested by an increase in the content of nutrients and chlorophyll in the ear leaf, that is considered a predictive organ for grain yield. The fertilizers, Super N-46 and UltraGran stabilo, had a positive effect on the chlorophyll content (CCI parameter) and increased its efficiency of excitation energy transfer (the F0 parameter).
The antioxidant and neuroprotective potential of leaves and inflorescences extracts of selected hemp varieties obtained with scCO2
Cannabis sativa, a versatile plant with numerous varieties, holds promising potential for a wide range of biological activity. As raw materials for research, we chose leaves and inflorescences of hemp varieties such as Białobrzeskie, Henola, and Tygra, which are cultivated mainly for their fibers or seeds. The choice of extraction is a key step in obtaining the selected compositions of active compounds from plant material. Bearing in mind the lipophilic nature of cannabinoids, we performed supercritical carbon dioxide (scCO2) extraction at 50 °C under 2000 (a) and 6000 PSI (b). The cannabinoid contents were determined with the use of the HPLC-DAD method. The antioxidant capabilities were assessed through a series of procedures, including the DPPH, ABTS, CUPRAC, and FRAP methods. The capacity to inhibit enzymes that play a role in the progression of neurodegenerative diseases, such as acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase was also studied. The dominant cannabinoids in the extracts were cannabidiol (CBD) and cannabidiolic acid (CBDA). The highest concentration of eight cannabinoids was detected in the Tygra inflorescences extract (b). The most notable antioxidant properties were provided by the Tygra inflorescences extract (b). Nonetheless, it was the Henola inflorescences extract (b) that demonstrated the most efficient inhibition of AChE and BChE, and tyrosinase was inhibited the most significantly by the Białobrzeskie inflorescences extract (b). Multidimensional comparative analysis enrolled all assays and revealed that the Henola inflorescences extract (b) showed the most substantial neuroprotective potential.
Antidiabetic Potential of Black Elderberry Cultivars Flower Extracts: Phytochemical Profile and Enzyme Inhibition
Black elderberry (Sambucus nigra L.) flowers are rich in polyphenolic compounds, including chlorogenic acid and quercetin derivatives, which are known for their health benefits, particularly their antioxidant and antidiabetic properties. This study aimed to optimize the extraction conditions using the Box–Behnken model to maximize polyphenol yields from different elderberry flower cultivars and to evaluate their potential for antidiabetic action. The extracts were analyzed for their phytochemical content and assessed for enzyme inhibition, specifically targeting enzymes critical in carbohydrate digestion and glucose regulation. The anti-inflammatory activity was also assessed. Results indicated that the Black Beauty, Obelisk, and Haschberg cultivars demonstrated significant inhibition of α-glucosidase, with a high inhibitory potential against α-amylase enzymes for the Obelisk cultivar. Additionally, high chlorogenic acid content was strongly correlated with enzyme inhibition and antioxidant activity, suggesting its substantial role in glucose regulation. This study underscores the potential of elderberry flower extracts, particularly those rich in chlorogenic acid, as natural agents for managing blood glucose levels, warranting further exploration of their use in antidiabetic applications.
Introdukcja uprawy imbiru (Zingiber officinale Rosc.) w Polsce na potrzebę opracowania nowej technologii wytwarzania klarowanych napojów funkcjonalnych. Grupa Operacyjna: Polski Imbir
Genotype-Trait (GT) Biplot Analysis for Yield and Quality Stability in Some Sweet Corn (Zea mays L. saccharata Sturt.) Genotypes
A strong statistical method for investigating the correlations between traits, assessing genotypes based on numerous traits, and finding individuals who excel in particular traits is genotype–trait (GT) biplot analysis. The current study was applied to evaluate 11 sweet corn (Zea mays L. saccharata) genotypes and correlate them based on genotype–trait (GT) biplot analysis for two cropping seasons in Erzurum, Türkiye using the RCBD experimental design with three reputations. The results showed that the genotypes were significantly different for the majority of the examined variables according to the combined analysis of variance findings at 0.01 probability level. An ecological analysis was performed to evaluate sweet corn varieties and environmental conditions and interactions between them (genotype × environmental conditions). Our results showed that the summation of the first two and second main components was responsible for 73.51% of the combined cropping years of the sweet corn growth and development variance, demonstrating the biplot graph’s optimum relative validity, which was obtained. In this study, the Khan F1 (G6) genotype was found to be the stablest genotype, and the Kompozit Seker (G7) genotype was the non-stable genotype, moreover based on the first cropping year, second cropping year, and the average mean of the two cropping years. As a conclusion, the Khan F1 (G6) genotype is the highest-yielding genotype, and the Kompozit Seker (G7) is the lowest. Based on the heat map dendrogram, the context of the differential extent of trait association of all genotypes into two clusters is indicated. The highest genetic distance was shown between the BATEM Tatlı (G3) and Febris (G5) genotypes. Our results provide helpful information about the sweet corn genotypes and environments for future breeding programs.
Exploring Beneficial Properties of Haskap Berry Leaf Compounds for Gut Health Enhancement
This study investigates the potential of formulated systems utilising haskap berry leaf extracts and dextran as carriers, to modulate both antioxidant and enzymatic inhibitory activities and their impact on the growth of specific bacterial strains. The analysis of antioxidant capacity, assessed through ABTS, CUPRAC, DPPH, and FRAP assays, revealed varying but consistently high levels across extracts, with Extract 3 (loganic acid: 2.974 mg/g, chlorogenic acid: 1.125 mg/g, caffeic acid: 0.083 mg/g, rutin: 1.137 mg/g, and quercetin: 1.501 mg/g) exhibiting the highest values (ABTS: 0.2447 mg/mL, CUPRAC: 0.3121 mg/mL, DPPH: 0.21001 mg/mL, and FRAP: 0.3411 mg/mL). Subsequent enzymatic inhibition assays demonstrated a notable inhibitory potential against α-glucosidase (1.4915 mg/mL, expressed as acarbose equivalent), hyaluronidase (0.2982 mg/mL, expressed as quercetin equivalent), and lipase (5.8715 µg/mL, expressed as orlistat equivalent). Further system development involved integration with dextran, showcasing their preserved bioactive compound content and emphasising their stability and potential bioactivity. Evaluation of the dextran systems’ impact on bacterial growth revealed a significant proliferation of beneficial strains, particularly the Bifidobacterium and lactobacilli genus (Bifidobacterium longum: 9.54 × 107 to 1.57 × 1010 CFU/mL and Ligilactobacillus salivarius: 1.36 × 109 to 1.62 × 1010 CFU/mL), suggesting their potential to modulate gut microbiota. These findings offer a foundation for exploring the therapeutic applications of haskap berry-based dextran systems in managing conditions like diabetes, emphasising the interconnected roles of antioxidant-rich botanical extracts and dextran formulations in promoting overall metabolic health.
Wpływ czynników agrotechnicznych w kontekście ograniczenia zawartości składników mineralnych w glebie po zbiorze kukurydzy
The Evaluation of Sweetcorn (Zea mays saccharata Sturt.) Infestation of Barnyardgrass (Echinochloa crus-galli) Depending on Weather Conditions and Crop Rotation
This paper focuses on the weed species Echinochloa crus-galli, commonly known as barnyardgrass, which is a persistent threat to crop yield and quality, especially in maize (Zea mays) cultivation. It is one of the most problematic weeds in agricultural fields due to its aggressive growth, adaptability to different environmental conditions and prolific seed production. The incidence of Echinochloa crus-galli in maize fields has increased in recent years. This study aims to provide a comprehensive understanding of the characteristics and behavior of Echinochloa crus-galli, and to suggest effective measures to control it. This research on sweetcorn was conducted from 1992 to 2019 at the Research and Education Center Gorzyń, Złotniki branch, which belongs to the Poznań University of Life Sciences. The evaluation of weed infestation was carried out in experiments focusing on chemical weed control in maize. The experiments were designed as a one-factor randomised block design with four field replications. The condition and the degree of weed infestation (number of weeds and fresh weight of weeds) in the control plots was assessed on an annual basis at the end of June and in July. The aim of the study was to evaluate the dynamic changes in the status and extent of barnyardgrass (Echinochloa crus-galli) infestation in maize grown after various other crops in the Wielkopolska region, with a focus on the weather conditions. The study found that barnyardgrass was most likely to occur when maize was sown after winter wheat in a dry and warm year, and least likely when maize was grown in rotation after winter wheat in a cold year with average rainfall. The proportion of barnyardgrass weed mass in the total weed mass was significantly lower after winter rye than after winter wheat, winter oilseed rape and winter triticale. Further research into the biology and ecology of barnyardgrass is key to effectively controlling this weed and safeguarding sweetcorn yields.
Preliminary Studies on the Effect of Soil Conditioner (AMP) Application on the Chemical and Microbiological Properties of Soil under Winter Oilseed Rape Cultivation
This study analyzed the effect of the application of a soil conditioner under the trade name of the Agro Mineral Product (AMP) in the winter rapeseed cultivation on the bacterial and fungal abundance, ion concentrations, and electrolytic conductivity of the soil solution. It was demonstrated that the AMP influenced changes in the total abundance of the culturable fractions of the soil bacteria and fungi at each of the tested time points. A stimulatory effect of the preparation on the growth of the soil bacteria and an inhibitory effect on the development of the fungi was observed, particularly at doses of 4 and 8 t·ha−1. A dose of 12 t·ha−1 proved to be the least effective in relation to the development of the soil microbiome. Increasing the AMP fertilization dose above 4 t·ha−1 caused changes in the chemistry of the soil solution (pH, EC, HCO3−, K+, and PO4-P). It is worth noting that this primarily resulted in decreases in the amounts of mobile forms of potassium (from 40.4 mg·dm−3 in the control to 26.7 mg·dm−3 at the 8 t·ha−1 dose) and orthophosphate as phosphorus (from −6.00 mg·dm−3 in the control to 3.75 mg·dm−3 at the 8 t·ha−1 dose) in the soil solution, which resulted in a reduction in the yield of the winter rapeseed (from 4.76 t·ha−1 in the control to 4.61 t·ha−1 at the 8 t·ha−1 and 4.43 t·ha−1 at the 12 t·ha−1 AMP dose).
Influence of Application Timings, Rates, and Adjuvants on Tiencarbazone-Methyl Plus Isoxaflutole and Mesotrione with Nicosulfuron and Rimsulfuron on Weed Control and Yield of Maize
Weed control in maize is usually limited to a single herbicide treatment, but the application of two or more herbicides is associated with many benefits, e.g., increasing the spectrum of control weeds, reducing the risk of damage to crops by using reduced rates of herbicides, limiting their residues in the soil or crop, etc. This field experiment was conducted in the years 2016–2018 to determine whether the split application of soil-applied thiencarbazone-methyl + isoxaflutole and foliar-applied mesotrione + nicosulfuron + rimsulfuron, in reduced rates with adjuvants, can contribute to enhancing herbicide effectiveness and increasing maize yield. Weed control in maize in a split-dose system with a mixture of thiencarbazone-methyl + isoxaflutole at strongly reduced rates with the addition of UAN and the adjuvant Atpolan SoilMaxx or Grounded, and then mesotrione + nicosulfuron + rimsulfuron at strongly reduced rates with UAN and Atpolan SoilMaxx or Actirob 842 EC allowed for great control of weeds. The total amount of substances was slightly higher than in single treatments with adjuvants, but lower than for individual active substances, leading to a reduction in the amount of active substances reaching the environment, while maintaining very high herbicide efficacy.
Effect of Maize Residual Nitrogen on Grain Yield and Composition of Subsequent Wheat Crops
Common wheat (Triticum aestivum ssp. vulgare) is one of the three basic cereal crops worldwide that plays a key role in global food security. A key factor affecting the yield and traits of common wheat is an adequate nitrogen supply. Improving the efficiency of soil nitrogen use can be achieved through the application of appropriate mineral fertilizers and the proper selection of cultivars. The aim of this study was to determine the impact of residual nitrogen (Nres) after maize cultivation (the preceding crop) on the yield and chemical composition of winter and spring wheat grain. It was shown that both the variety selection and the type of nitrogen carrier had a significant impact on the characteristics related to wheat yield and grain quality. The most stable effect of the type of nitrogen, regardless of the type of corn variety, was recorded for ammonium nitrate with N-Lock. The average yield was approximately 6.1 t ha−1. With the exception of the variant with N-Lock, the most progressive reaction to the type of fertilizer occurred in the stand with a three-line corn hybrid (TC, stay green). The advantage of this corn variety as a winter wheat forecrop results from the value of the site in a site without nitrogen. In the nitrogen control, the increase in yield compared to the single corn hybrid (SC) was 14%. However, in the U + N-Lock variant, it was 17%, and SG Stabilo as much as 32%. The increase in the weight of 1000 wheat grains in the stands after the SC and TC hybrid compared to stay green + roots power indicates a compensatory mechanism that became visible in the grain filling phase. Current challenges in agriculture caused by population growth and the need to ensure sufficient food production require greater awareness and knowledge regarding improved nitrogen management, including recognizing the role of residual nitrogen remaining in the soil after the preceding crop. A major advantage of slow-release fertilizers is that the nutrient (N) is released in response to the dynamic demand of the crop. This, on the one hand, increases grain yield and, on the other, does not negatively impact the agrosystem (eutrophication).
