Filters
The community of soil fungi associated with the western red cedar (Thuja plicata Donn ex D. Don, 1824)
ABSTRACT The western red cedar (Thuja plicata Donn ex D. Don), an important forest-forming species in the Western part of the North American continent, is an alien species naturalised in Europe. It is popular and highly valued in horticulture. While considering the progressing climate change, it may also be a potential alternative to native species in European forests. The community of soil fungi associated with the western red cedar in forested areas of Europe has not been fully determined. Thus, this study is aimed to identify the community of soil fungi associated with the western red cedar. The experimental plots are located in the Kościan Forest District (51°98’87″ N; 16°23’54″ E). All soil samples were taken from the topsoil layer at a depth of 25 cm with a trowel, three from the centre of natural regeneration (1G, 2G, 5G) and three from the centre stand under the canopy of old-growth western red cedar (3G, 4G, 6G). Fungi were identified directly from the soil based on the ITS1 rDNA region. The derived product was sequenced using Illumina’s sequencing by synthesis (SBS) technology. Sequences were referred to the National Center for Biotechnology Information (NCBI) database applying the BLAST algorithm. The fungal counts were defined based on the number of operational taxonomic units (OTU) in the sample. The OTU number was 835 206, with fungal isolates accounting for 683 095 (81.79%). A total of 8 591 taxa belonging to the Kingdom Fungi were identified. The species with the greatest shares in the community included Mortierella spp. (10.5%), Russula spp. (5.6%), Hydnum spp. (3.44%), Solicoccozyma spp. (3.1%) and Penicillium spp. (2.2%). Results showed that saprotrophs and mycorrhizal fungi predominated in the community. The dominance of ectomycorrhizal fungi over arbuscular ones, quite impressive natural regeneration was shown in T. plicata stands in Kościan. Subsequent research should take into account tree stands in Poland in which natural regeneration does not occur or occurs sporadically.
Shields against pollution: phytoremediation and impact of particulate matter on trees at Wigry National Park, Poland
Enhancing Sustainability and Antifungal Properties of Biodegradable Composites: Caffeine-Treated Wood as a Filler for Polylactide
This study investigates the suitability of using caffeine-treated and untreated black cherry (Prunus serotina Ehrh.) wood as a polylactide filler. Composites containing 10%, 20%, and 30% filler were investigated in terms of increasing the nucleating ability of polylactide, as well as enhancing its resistance to microorganisms. Differential scanning calorimetry studies showed that the addition of caffeine-treated wood significantly altered the crystallization behavior of the polymer matrix, increasing its crystallization temperature and degree of crystallinity. Polarized light microscopic observations revealed that only the caffeine-treated wood induced the formation of transcrystalline structures in the polylactide. Incorporation of the modified filler into the matrix was also responsible for changes in the thermal stability and decreased hydrophilicity of the material. Most importantly, the use of black cherry wood treated with caffeine imparted antifungal properties to the polylactide-based composite, effectively reducing growth of Fusarium oxysporum, Fusarium culmorum, Alternaria alternata, and Trichoderma viride. For the first time, it was reported that treatment of wood with a caffeine compound of natural origin alters the supermolecular structure, nucleating abilities, and imparts antifungal properties of polylactide/wood composites, providing promising insights into the structure-properties relationship of such composites.
Variability of Functional Groups of Rhizosphere Fungi of Norway Spruce (Picea abies (L.) H.Karst.) in the Boreal Range: The Wigry National Park, Poland
Rhizosphere microbial communities can influence plant growth and development. Natural regeneration processes take place in the tree stands of protected areas, which makes it possible to observe the natural changes taking place in the rhizosphere along with the development of the plants. This study aimed to determine the diversity (taxonomic and functional) of the rhizosphere fungal communities of Norway spruce growing in one of four developmental stages. Our research was based on the ITS region using Illumina system sequencing. Saprotrophs dominated in the studied rhizospheres, but their percentage share decreased with the age of the development group (for 51.91 from 43.13%). However, in the case of mycorrhizal fungi, an opposite trend was observed (16.96–26.75%). The most numerous genera were: saprotrophic Aspergillus (2.54–3.83%), Penicillium (6.47–12.86%), Pyrenochaeta (1.39–11.78%), pathogenic Curvularia (0.53–4.39%), and mycorrhizal Cortinarius (1.80–5.46%), Pseudotomentella (2.94–5.64%) and Tomentella (4.54–15.94%). The species composition of rhizosphere fungal communities was favorable for the regeneration of natural spruce and the development of multi-generational Norway spruce stands. The ratio of the abundance of saprotrophic and mycorrhizal fungi to the abundance of pathogens was high and promising for the durability of the large proportion of spruce in the Wigry National Park and for forest ecosystems in general.
Climate warming, ecological dynamics and nature conservation drive tree diversity in Wigierski National Park, Poland
In response to ongoing climate warming, tree species adapted to colder climates are expected to shift their geographic ranges northward. Within the framework of long-term ecological monitoring in Wigry National Park (northeastern Poland), observed changes in forest biocenoses reflect the combined influence of climate change and natural ecological dynamics. This study compares dendroflora composition and diversity between two monitoring periods, 2011 and 2024, as part of an ongoing effort to track climate-related ecological shifts. Tree observations and measurements were carried out using concentric circular plots. In the largest plots, all trees with a diameter at breast height (d.b.h.) ≥ 12 cm were recorded by species, and their d.b.h. was measured. In the smaller plots, all trees with a d.b.h. ≥ 2 cm and < 2 cm but taller than 30 cm were similarly identified and measured. Data were recorded with Field-Map software integrated with an electronic calliper. The species-level taxonomic data, individual counts and basal area per species and plot were used to calculate biodiversity indices. Over the 13-year interval, a marked increase in overall dendroflora diversity was observed. Notably, the dominance of canopy-forming conifers – Pinus sylvestris and, to a lesser extent, Picea abies – measured as the proportion of individuals or stem density, has declined. This decline of coniferous species has been accompanied by an increase in the abundance and diversity of broadleaved deciduous species, including Tilia cordata, Quercus robur, Betula pendula, and Acer platanoides. Other thermophilous deciduous taxa also exhibited upward trends in both presence and abundance. Furthermore, the exponential of Shannon entropy, reached the highest value when evergreen conifers comprised 35% of the stand composition in 2011 and 18% in 2024. This finding suggests that maximum dendroflora diversity reaches its highest level at an intermediate proportion of conifers presence, rather than under conifers dominance or absence. Collectively, the processes occurring in Wigierski National Park illustrate the gradual shift in ecotonal forest ecosystems from cold-adapted coniferous species to broadleaved deciduous taxa due to ongoing climate change.
Loranthus europaeus - A New Challenge for Silviculture and Protection of Deciduous Stands in Poland?
Walory użytkowe krzewów liściastych – dereni, głogów i irg w zadrzewieniach i systemach rolno-leśnych
The use of attractants in reducing the occurrence of the Prunus serotina (Ehrh.)
Abstract Black cherry, which occurs in European forests, contributes to their distortion or degradation of native ecosystems. Therefore, measures are taken to reduce its occurrence. One of them is the biological method involving grazing animals. The aim of this research was to develop and identify an attractant that would increase the attractiveness of black cherry as a feeding base for deer, which would limit its occurrence. This research was carried out in Zielonka (17.10941 E 52.553975 N). In the experiment, 3-year-olds were used: Tilia cordata, Acer pseudoplatanus, A. platanoides, Fagus sylvatica and Prunus serotina. Before planting the seedlings, their height was measured. The control (K, 10 trees) consisted of trees of each species that were not sprayed with attractants. Another set of 10 trees were treated over the entire surface of the shoots with a bait mixture (MW), and the next 10 trees were sprayed with a 10% aqueous solution of NaCl. The attractant spraying was repeated six times. All damage observed was recorded. The inventoried tree damage was divided into four categories: 0%, no damage; I, 1–20% damaged trees; II, 21–50% damaged trees and III, more than 50% damaged trees. The results of the Mann–Whitney U test (p = 0.0109), at the assumed level of significance (α = 0.05), showed that slightly higher seedlings were browsed. These studies indicate the potential of using attractants as preparations influencing the palatability of the black cherry for deer. All recorded bites were classified as degree I damage. The trees were gnawed by fallow deer, 35.33% of trees were not damaged by game at all, and 64.66% of trees were damaged in degree I. In the case of sycamore maple, Norway maple and linden, the bait mixture did not increase the number of nibbled buds and leaves, while NaCl increased the attractiveness of beech and black cherry as a feeding base. Conducting further research studies on the improvement of preparations may give a chance to reduce the use of chemicals in forest protection and give the possibility of using attractants where it is impossible to use herbicides.
Silver fir restitution: The role of seedling stock type in adapting to various environmental conditions
Cherry spruce rust in the Wigry National Park and Suwałki Forest District: cone infestation and its implications
Abstract Thekopsora areolata and Chrysomyxa pirolata are pathogens preferentially infesting Norway spruce cones and lead to the complete failure of spruce seed yield. Thus, the presence of seed-injuring pathogens may be a crucial factor driving close association between cherry spruce rust occurrence on trees and limited natural regeneration of the preferred host. However, a major uncertainty remains as to which pathogens are responsible for cone infection and reduced seed viability, resulting in impeded renewal of young trees. Our study aims to investigate whether T. areolata or C. pirolata presence on the Norway spruce cones was responsible for cherry spruce rust in three sites within the Wigry National Park (one site) and the Suwałki Forest District (two sites) in North-Eastern Poland. We harvested randomly five infested Norway spruce cones from each location and analysed them to verify specific pathogen occurrence. We analysed rDNA fragments such as ITS regions and 28S to precisely identify the disease causal agent on the cones and estimate the potential of genetic diversity of the analysed fungus species. Thekopsora areolata was identified as the main fungus promoting cherry spruce rust, suggesting that host species regeneration is constrained by this pathogen’s distribution. We assumed that the apparent cherry spruce rust presence and lack of Norway spruce natural regeneration likely result from cone infection and the availability of healthy, high-quality seeds. Moreover, the occurrence of cherry spruce rust may explain the challenges associated with the deficit of seeds used for the artificial regeneration of spruce stands.
Prospects for Oak Cultivation in Europe Under Changing Environmental Conditions and Increasing Pressure from Harmful Organisms
It is assumed that climate change (global warming) worsens the living conditions for conifers and at the same time favours the cultivation of deciduous trees, including oaks. In fact, in Poland, for example, many more oaks are now being planted as forest-forming tree species than in the 1980s and 1990s. However, the monitoring of the health status of European forests (according to the International Co-operation Project) does not confirm these optimistic assumptions, and oak has been cited as one of the most damaged tree species in terms of defoliation in recent decades. The prospects for oak cultivation in European forestry are therefore a combination of abiotic conditions and biotic damage factors. This review article focuses in particular on the new threats posed by pathogenic organisms causing emerging diseases. These include newly identified bacteria responsible for the so-called Acute Oak Decline (AOD), oomycetes (especially those specialised in damaging fine roots, such as Phytophthora quercina T.Jung) and semi-parasites of the genus Loranthus. At the same time, the pressure from commonly observed insects and fungi described in connection with the complex syndrome of oak decline, which is divided into predisposing, inciting, and contributing factors (according to Manion’s disease spiral), has not abated. Therefore, international, interdisciplinary research (such as that proposed in Oakland) is needed, using modern technologies (RS remote sensing) based on the comparison of satellite images (from different years), not only to inventory the most valuable oak stands in Europe (microrefugia) but also to identify trends in changes in their condition and biodiversity. As RS has its limitations (e.g., resolution), aerial monitoring should be complemented by quantitative and qualitative inventory from the ground, e.g., monitoring of the presence of soil microorganisms using effective molecular biological methods (e.g., Next-Generation Sequencing NGS).
The Community of Fine Root Fungi of Silver Fir (Abies alba Mill.) Saplings
This study aimed to assess the biodiversity of fungi colonizing the fine roots (diameter up to 2 mm) of 3-year-old silver fir saplings from areas of Międzylesie Forest District in Poland. It was hypothesized that quantitatively and qualitatively, mycorrhizal fungi would be the dominant fungi in root communities of silver fir. DNA extraction was performed using Plant Genomic DNA purification. The internal transcribed spacer1 (ITS1) rDNA region was amplified using specific primers, and the amplicons were purified and sequenced using sequencing by synthesis (SBS) Illumina technology. The obtained sequences were compared with reference sequences in the UNITE database (https://unite.ut.ee/) using the basic local alignment search tool (BLAST) algorithm to facilitate species identification. A total of 307,511 OTUs was obtained from each sample. There were 246,477 OTUs (80.15%) of fungi known from cultures. The genera Tuber spp. (7.51%) and Acephala spp. (3.23%) accounted for the largest share of the fungal communities on the fine roots of fir trees. Hence our results indicate the dominance of mycorrhizal fungi in these communities and reflect the excellent quality of the saplings that were assessed. Pathogenic fungi constituted a much smaller share of the fungal communities.
Influence of endozoochory and stress conditions on the germination of black cherry Prunus serotina Ehrh. seeds outside the natural range
Assessment of sodium chloride application for controlling invasive black cherry Prunus serotina Ehrh.
Predicting parasitic plants Loranthus Europaeus range shifts in response to climate change
Abstract Climate change significantly influences the distribution of parasitic species, posing threats to ecosystems and economies. This study examines the potential range expansion of Loranthus europaeus, a parasitic plant impacting European forestry. We assessed the impact of predicted climate change for 2041–2060 and 2061–2080 using MaxEnt modeling based on current occurrence data of L. europaeus, and the main host plant genus oak Quercus, as well as bioclimatic variables. Our model demonstrated high predictive accuracy (AUC = 0.92). The most important variables for Europe range were range of Quercus genus. Key environmental factors included isothermality (bio3) and mean temperature of wettest quarter (bio8). Under SSP126 and SSP245 scenarios, our results predict significant range expansions into northern and eastern Europe, with increases of 43.5% and 53.9% by 2041–2060. Conversely, southern Europe may see contractions of 16.4–20.6%. Projections for 2061–2080 indicate further expansions up to 65.8% in northern Europe, alongside contractions up to 29.8% in southern regions, including Turkey and Greece.These shifts highlight the influence of climate change on L. europaeus distribution and underscore the need for adaptive management strategies to mitigate potential ecological and economic impacts.
