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Expression patterns of candidate genes for the Lr46/Yr29 “slow rust” locus in common wheat (Triticum aestivum L.) and associated miRNAs inform of the gene conferring the Puccinia triticina resistance trait

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dc.abstract.enLeaf rust caused by Puccinia triticina (Pt) is one of the most impactful diseases causing substantial losses in common wheat (Triticum aestivum L.) crops. In adult plants resistant to Pt, a horizontal adult plant resistance (APR) is observed: APR protects the plant against multiple pathogen races and is distinguished by durable persistence under production conditions. The Lr46/Yr29 locus was mapped to chromosome 1B of common wheat genome, but the identity of the underlying gene has not been demonstrated although several candidate genes have been proposed. This study aimed to analyze the expression of nine candidate genes located at the Lr46/Yr29 locus and their four complementary miRNAs (tae-miR5384-3p, tae-miR9780, tae-miR9775, and tae-miR164), in response to Pt infection. The plant materials tested included five reference cultivars in which the molecular marker csLV46G22 associated with the Lr46/Yr29-based Pt resistance was identified, as well as one susceptible control cultivar. Biotic stress was induced in adult plants by inoculation with fungal spores under controlled conditions. Plant material was sampled before and at 6, 12, 24, 48 hours post inoculation (hpi). Differences in expression of candidate genes at the Lr46/Yr29 locus were analyzed by qRT-PCR and showed that the expression of the genes varied at the analyzed time points. The highest expression of Lr46/Yr29 candidate genes (Lr46-Glu1, Lr46-Glu2, Lr46-Glu3, Lr46-RLK1, Lr46-RLK2, Lr46-RLK3, Lr46-RLK4, Lr46-Snex, and Lr46-WRKY) occurred at 12 and 24 hpi and such expression profiles were obtained only for one candidate gene among the nine genes analyzed (Lr46-Glu2), indicating that it may be a contributing factor in the resistance response to Pt infection.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliationKatedra Metod Matematycznych i Statystycznych
dc.affiliation.instituteKatedra Genetyki i Hodowli Roślin
dc.affiliation.instituteKatedra Technologii Żywności Pochodzenia Roślinnego
dc.affiliation.instituteKatedra Metod Matematycznych i Statystycznych
dc.affiliation.instituteKatedra Biochemii i Biotechnologii
dc.contributor.authorSpychała, Julia
dc.contributor.authorTomkowiak, Agnieszka
dc.contributor.authorNoweiska, Aleksandra
dc.contributor.authorBobrowska, Roksana
dc.contributor.authorRychel-Bielska, Sandra
dc.contributor.authorBocianowski, Jan
dc.contributor.authorWolko, Łukasz
dc.contributor.authorKowalczewski, Przemysław Łukasz
dc.contributor.authorNowicki, Marcin
dc.contributor.authorKwiatek, Michał Tomasz
dc.date.access2024-09-16
dc.date.accessioned2024-10-11T09:52:10Z
dc.date.available2024-10-11T09:52:10Z
dc.date.copyright2024-09-06
dc.date.issued2024
dc.description.abstract<jats:p>Leaf rust caused by <jats:italic>Puccinia triticina</jats:italic> (<jats:italic>Pt</jats:italic>) is one of the most impactful diseases causing substantial losses in common wheat (<jats:italic>Triticum aestivum</jats:italic> L.) crops. In adult plants resistant to <jats:italic>Pt</jats:italic>, a horizontal adult plant resistance (APR) is observed: APR protects the plant against multiple pathogen races and is distinguished by durable persistence under production conditions. The <jats:italic>Lr46/Yr29</jats:italic> locus was mapped to chromosome 1B of common wheat genome, but the identity of the underlying gene has not been demonstrated although several candidate genes have been proposed. This study aimed to analyze the expression of nine candidate genes located at the <jats:italic>Lr46/Yr29</jats:italic> locus and their four complementary miRNAs (tae-miR5384-3p, tae-miR9780, tae-miR9775, and tae-miR164), in response to <jats:italic>Pt</jats:italic> infection. The plant materials tested included five reference cultivars in which the molecular marker <jats:italic>csLV46G22</jats:italic> associated with the <jats:italic>Lr46/Yr29</jats:italic>-based <jats:italic>Pt</jats:italic> resistance was identified, as well as one susceptible control cultivar. Biotic stress was induced in adult plants by inoculation with fungal spores under controlled conditions. Plant material was sampled before and at 6, 12, 24, 48 hours post inoculation (hpi). Differences in expression of candidate genes at the <jats:italic>Lr46/Yr29</jats:italic> locus were analyzed by qRT-PCR and showed that the expression of the genes varied at the analyzed time points. The highest expression of <jats:italic>Lr46/Yr29</jats:italic> candidate genes (<jats:italic>Lr46-Glu1</jats:italic>, <jats:italic>Lr46-Glu2</jats:italic>, <jats:italic>Lr46-Glu3</jats:italic>, <jats:italic>Lr46-RLK1</jats:italic>, <jats:italic>Lr46-RLK2</jats:italic>, <jats:italic>Lr46-RLK3</jats:italic>, <jats:italic>Lr46-RLK4</jats:italic>, <jats:italic>Lr46-Snex</jats:italic>, and <jats:italic>Lr46-WRKY</jats:italic>) occurred at 12 and 24 hpi and such expression profiles were obtained only for one candidate gene among the nine genes analyzed (<jats:italic>Lr46-Glu2</jats:italic>), indicating that it may be a contributing factor in the resistance response to <jats:italic>Pt</jats:italic> infection.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,9
dc.description.number9
dc.description.points100
dc.description.versionfinal_published
dc.description.volume19
dc.identifier.doi10.1371/journal.pone.0309944
dc.identifier.issn1932-6203
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/1830
dc.identifier.weblinkhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0309944
dc.languageen
dc.relation.ispartofPLoS ONE
dc.relation.pagese0309944
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.titleExpression patterns of candidate genes for the Lr46/Yr29 “slow rust” locus in common wheat (Triticum aestivum L.) and associated miRNAs inform of the gene conferring the Puccinia triticina resistance trait
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue9
oaire.citation.volume19